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油菜脅迫蛋白SAP家族成員挖掘與鑒定

時間:2019-04-14 13:38來源:畢業論文
非生物脅迫如寒害、旱害、鹽害、病蟲害等對植物的生長發育具有較大程度的危害,而嚴重的非生物脅迫對油菜等作物會造成嚴重的經濟損失,從而影響作物的生產。植物在非生物脅迫下

摘  要:非生物脅迫如寒害、旱害、鹽害、病蟲害等對植物的生長發育具有較大程度的危害,而嚴重的非生物脅迫對油菜等作物會造成嚴重的經濟損失,從而影響作物的生產。植物在非生物脅迫下,會誘導產生多種蛋白以適應脅迫壓力,其中 SAP(stress-associated protein) 蛋白在脅迫應答和脅迫調控中具有重要作用而被廣泛關注。因此不斷挖掘與鑒定新的脅迫相關蛋白是植物學研究的熱點之一。為了獲取油菜中脅迫相關的蛋白信息,本研究對 NCBI蛋白質序列數據庫進行檢索獲得27條白菜型油菜脅迫相關蛋白序列,并運用生物信息學技術及方法對該家族蛋白序列的基本信息、結構、定位、蛋白序列保守域和進化信息等進行了分析。結果表明,油菜SAP蛋白家族共27條序列(除去一致性序列),分布在第Ⅰ至Ⅸ條染色體上,其中第9號染色體上SAP基因位點最多,為7個基因位點,基因位點最少的染色體是1號和4號染色體;所有蛋白序列成員中19個定位于細胞核中,有3個成員定位于細胞外,5條同時定位于細胞核和線粒體中;該家族成員對應基因具有1-4個外顯子;按保守結構域不同將其分為三類,其中第一類4個成員,具有RAMP4結構域特征,第二類1個成員,具有zfA20結構域系列特征,第三類分別具有22個成員,同時具有ZnF-AN1和zfA20結構域序列特征。進化分析表明,SAP蛋白具有較多異變位點,多序列比對的差異性大,在進化過程中易發生基因突變來適應脅迫環境。本研究為利用相關數據開發油菜的相關抗逆新品種奠定理論基礎。34529
畢業論文關鍵詞:油菜;SAP蛋白;基因結構;進化分析

源`自*六)維[論*文'網www.mmeqir.tw


Discovery and Identification of Stress-associated Protein of SPA Family Members in Brassica Rape L
Abstract: Abiotic stresses such as chilling injury, damage of drought, salinity and plant diseases and insect pests, is harmful to plant growth and development. Greater degree of harm and serious abiotic stress will be caused serious economic losses on rape and other crops, which affecting crop production. With abiotic stresse, crops would be induced to produce varieties of protein to adapt to the stress pressure, the SAP (stress-associated protein) proteins induced by abiotc stress have important roles on responses of stress and the regulation of stress. Therefore, it is one of the hot topics in botanical research to tap and identify new stress-related proteins. In order to obtain the information of stress-associated protein of rape. This study were retrieved 27 different stress-associated protein sequences of cabbage from NCBI protein sequence database, then by biological information technology and the methods,  we got basic physical and chemical information, structure information, location, protein sequences conserved domains and evolutionary information. The results showed that, the SAP protein family in rape has 27 diferent members (excluding consistent sequences), distributed in from I to IX chromosomes, the number of SAP gene sites on ninth chromosome has 7 genetic locus, the genetic locus in the 1th and 4th chromosome was the fewest. In all SAP stress-association protein members, 19 members located in the nucleus, 3 members located in extracellular, 5 members located in both nucleus and mitochondria; the family members have 1-4 exons; according to the different conserved domains, it will be pided into three categories, the first category has 4 members, which have RAMP4 domain, the second only has 1 member, with the zfA20 domain, the third type have 22 members, with both ZnF-AN1 domain and zfA20 domain. Evolutionary analysis showed that the SAP protein had many mutation sites, and the difference of multiple sequence alignment was larger, and the gene mutation was prone to adapt to the stress environment during the evolution. This research is to lay the theoretical foundation for the development of rape new varieties– resisitance to stress. 油菜脅迫蛋白SAP家族成員挖掘與鑒定:http://www.mmeqir.tw/shengwu/20190414/32052.html
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